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pet30a his6-tag) vector  (Millipore)


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    Structured Review

    Millipore pet30a his6-tag) vector
    Pet30a His6 Tag) Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet30a+vector/pet+15b/pm38390831-287-12-18
    Average 90 stars, based on 1 article reviews
    pet30a his6-tag) vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Elicitation of potent neutralizing antibodies in obese mice by ISA 51-adjuvanted SARS-CoV-2 spike RBD-Fc vaccine
    Article Snippet: .. The pET30a vector (Novagen, Merck Group) encoding for the RBD fused with an N-terminal superfolder GFP (sfGFP) fusion tag (Pedelacq et al. ) and a C-terminal His tag was used as the template for site-directed mutagenesis. .. The amino acid residues 442–460 and 473–495 in the RBD were individually replaced with alanine by using the PCR-based site-directed mutagenesis method as described in the previous study (Lai et al. ).

    Article Title: Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
    Article Snippet: .. A synthetic gene coding for M. hassiacum MmpH and sequence-optimized for expression in E. coli (GenScript) was cloned into the NdeI and HindIII restriction sites of the pET30a vector (Novagen) and C-terminal His6-tagged MmpH was overexpressed in E. coli BL21(DE3) cells. ..

    Article Title: Elicitation of potent neutralizing antibodies in obese mice by ISA 51-adjuvanted SARS-CoV-2 spike RBD-Fc vaccine.
    Article Snippet: .. The pET30a vector (Novagen, Merck Group) encoding for the RBD fused with an N-terminal superfolder GFP (sfGFP) fusion tag (Pedelacq et al. 2006) and a C-terminal His tag was used as the template for site-directed mutagenesis. .. The amino acid residues 442–460 and 473–495 in the RBD were individually replaced with alanine by using the PCR-based site-directed mutagenesis method as described in the previous study (Lai et al. 2022).

    Article Title: Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
    Article Snippet: .. A synthetic gene coding for M. hassiacum MeT1 was sequenceoptimized (GenScript) for expression in E. coli, cloned into the NdeI and HindIII restriction sites of the pET30a vector (Novagen), and overexpressed in E. coli BL21(DE3) cells as a C-terminal His6-tagged MeT1. ..

    Article Title: Protein prostheses for mitochondrial diseases or conditions
    Article Snippet: Ferric 0.0001 0.0001 0.0001 0.0001 0.0001 Nitrate•9H2O Magnesium 0.09767 0.09767 0.09767 0.09767 0.09767 Sulfate Potassium 0.4 0.4 0.4 0.4 0.4 Chloride Sodium Chloride 6.4 6.4 6.4 6.4 6.4 Sodium Phosphate 0.109 0.109 0.109 0.125 NaH2PO4—H2O 0.125 NaH2PO4—H2O Monobasic Sodium Bicarbonate Absent Absent Absent 3.7 3.7 (NaHCO3) Amino Acids: L-Arginine•HCl 0.084 0.084 0.084 0.084 0.084 L-Cystine•2HCl 0.0626 0.0626 0.0626 0.063 0.063 L-Glutamine 0.584 Absent 0.584 0.584 Absent Glycine 0.03 0.03 0.03 0.03 0.03 L-Histidine•HCl•H2O 0.042 0.042 0.042 0.042 0.042 L-Isoleucine 0.105 0.105 0.105 0.105 0.105 L-Leucine 0.105 0.105 0.105 0.105 0.105 L-Lysine•HCl 0.146 0.146 0.146 0.146 0.146 L-Methionine 0.03 0.03 0.03 0.03 0.03 L-Phenylalanine 0.066 0.066 0.066 0.066 0.066 L-Serine 0.042 0.042 0.042 0.042 0.042 L-Threonine 0.095 0.095 0.095 0.095 0.095 L-Tryptophan 0.016 0.016 0.016 0.016 0.016 L-Tyrosine•2Na•2H2O 0.10379 0.10379 0.10379 0.104 0.104 L-Valine 0.094 0.094 0.094 0.094 0.094 Vitamins: Choline Chloride 0.004 0.004 0.004 0.004 0.004 Folic Acid 0.004 0.004 0.004 0.004 0.004 myo-Inositol 0.0072 0.0072 0.0072 0.0072 0.0072 Niacinamide 0.004 0.004 0.004 0.004 0.004 D-Pantothenic 0.004 0.004 0.004 0.004 0.004 Acid, Ca Pyridoxal•HCl 0.004 0.004 0.004 0.004 0.004 Riboflavin 0.0004 0.0004 0.0004 0.0004 0.0004 Thiamine•HCl 0.004 0.004 0.004 0.004 0.004 Other: D-Glucose 1 Absent 1 4.5 Absent Pyruvic Acid, 0.11 0.11 Absent 0.11 Absent Sodium Phenol Red, 0.0159 0.0159 Absent 0.015 Absent Sodium Cloning of NOXes into pLVX-TRE3G Vector Human codon optimized genes encoding H2O-forming NADH oxidases (NOXes) from Lactobacillus brevis (SEQ ID NO: 316), Lactococcus lactis (SEQ ID NO: 317), Streptococcus pneumoniae (SEQ ID NO: 318), Streptococcus mutans (SEQ ID NO: 319), and Serpulina hyodysenteriae (SEQ ID NO: 320), flanked by NotI and MluI restriction sites in pUC57 vector, were custom synthesized by GENEWIZ. .. After digestion PCR product was ligated into pET30a vector (EMD Millipore). ..

    Mutagenesis:

    Article Title: Elicitation of potent neutralizing antibodies in obese mice by ISA 51-adjuvanted SARS-CoV-2 spike RBD-Fc vaccine
    Article Snippet: .. The pET30a vector (Novagen, Merck Group) encoding for the RBD fused with an N-terminal superfolder GFP (sfGFP) fusion tag (Pedelacq et al. ) and a C-terminal His tag was used as the template for site-directed mutagenesis. .. The amino acid residues 442–460 and 473–495 in the RBD were individually replaced with alanine by using the PCR-based site-directed mutagenesis method as described in the previous study (Lai et al. ).

    Article Title: Elicitation of potent neutralizing antibodies in obese mice by ISA 51-adjuvanted SARS-CoV-2 spike RBD-Fc vaccine.
    Article Snippet: .. The pET30a vector (Novagen, Merck Group) encoding for the RBD fused with an N-terminal superfolder GFP (sfGFP) fusion tag (Pedelacq et al. 2006) and a C-terminal His tag was used as the template for site-directed mutagenesis. .. The amino acid residues 442–460 and 473–495 in the RBD were individually replaced with alanine by using the PCR-based site-directed mutagenesis method as described in the previous study (Lai et al. 2022).

    Sequencing:

    Article Title: Hfe Permease and Haemophilus influenzae Manganese Homeostasis.
    Article Snippet: Haemophilus influenzae is a commensal of the human upper respiratory tract that can infect diverse host niches due, at least in part, to its ability to withstand both endogenous and host-mediated oxidative stresses.. Here, we show that hfeA, a gene previously linked to iron import, is essential for H. influenzae manganese recruitment via the HfeBCD transporter.. Structural analyses show that metal binding in HfeA uses a unique mechanism that involves substantial rotation of the C-terminal lobe of the protein.

    Article Title: Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
    Article Snippet: .. A synthetic gene coding for M. hassiacum MmpH and sequence-optimized for expression in E. coli (GenScript) was cloned into the NdeI and HindIII restriction sites of the pET30a vector (Novagen) and C-terminal His6-tagged MmpH was overexpressed in E. coli BL21(DE3) cells. ..

    Article Title: A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’
    Article Snippet: .. The coding sequence of LoAAT1 was amplified using high-fidelity DNA polymerase KOD-Plus (TOYOBO) with the primers in and then constructed into the pET30a vector (Novagen) through the cloning sites Eco RI and Hind III. ..

    Amplification:

    Article Title: Hfe Permease and Haemophilus influenzae Manganese Homeostasis.
    Article Snippet: Haemophilus influenzae is a commensal of the human upper respiratory tract that can infect diverse host niches due, at least in part, to its ability to withstand both endogenous and host-mediated oxidative stresses.. Here, we show that hfeA, a gene previously linked to iron import, is essential for H. influenzae manganese recruitment via the HfeBCD transporter.. Structural analyses show that metal binding in HfeA uses a unique mechanism that involves substantial rotation of the C-terminal lobe of the protein.

    Article Title: A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’
    Article Snippet: .. The coding sequence of LoAAT1 was amplified using high-fidelity DNA polymerase KOD-Plus (TOYOBO) with the primers in and then constructed into the pET30a vector (Novagen) through the cloning sites Eco RI and Hind III. ..

    Article Title: A genetically encoded tool to increase cellular NADH/NAD + ratio in living cells.
    Article Snippet: .. For protein characterization studies E. coli sth gene was amplified from the pLVX-TRE3G vector using primers containing the BamHI and XhoI restriction sites and ligated into the pET30a vector (EMD Millipore) (Supplementary Table 5). ..

    Polymerase Chain Reaction:

    Article Title: Hfe Permease and Haemophilus influenzae Manganese Homeostasis.
    Article Snippet: Haemophilus influenzae is a commensal of the human upper respiratory tract that can infect diverse host niches due, at least in part, to its ability to withstand both endogenous and host-mediated oxidative stresses.. Here, we show that hfeA, a gene previously linked to iron import, is essential for H. influenzae manganese recruitment via the HfeBCD transporter.. Structural analyses show that metal binding in HfeA uses a unique mechanism that involves substantial rotation of the C-terminal lobe of the protein.

    Article Title: Protein prostheses for mitochondrial diseases or conditions
    Article Snippet: Ferric 0.0001 0.0001 0.0001 0.0001 0.0001 Nitrate•9H2O Magnesium 0.09767 0.09767 0.09767 0.09767 0.09767 Sulfate Potassium 0.4 0.4 0.4 0.4 0.4 Chloride Sodium Chloride 6.4 6.4 6.4 6.4 6.4 Sodium Phosphate 0.109 0.109 0.109 0.125 NaH2PO4—H2O 0.125 NaH2PO4—H2O Monobasic Sodium Bicarbonate Absent Absent Absent 3.7 3.7 (NaHCO3) Amino Acids: L-Arginine•HCl 0.084 0.084 0.084 0.084 0.084 L-Cystine•2HCl 0.0626 0.0626 0.0626 0.063 0.063 L-Glutamine 0.584 Absent 0.584 0.584 Absent Glycine 0.03 0.03 0.03 0.03 0.03 L-Histidine•HCl•H2O 0.042 0.042 0.042 0.042 0.042 L-Isoleucine 0.105 0.105 0.105 0.105 0.105 L-Leucine 0.105 0.105 0.105 0.105 0.105 L-Lysine•HCl 0.146 0.146 0.146 0.146 0.146 L-Methionine 0.03 0.03 0.03 0.03 0.03 L-Phenylalanine 0.066 0.066 0.066 0.066 0.066 L-Serine 0.042 0.042 0.042 0.042 0.042 L-Threonine 0.095 0.095 0.095 0.095 0.095 L-Tryptophan 0.016 0.016 0.016 0.016 0.016 L-Tyrosine•2Na•2H2O 0.10379 0.10379 0.10379 0.104 0.104 L-Valine 0.094 0.094 0.094 0.094 0.094 Vitamins: Choline Chloride 0.004 0.004 0.004 0.004 0.004 Folic Acid 0.004 0.004 0.004 0.004 0.004 myo-Inositol 0.0072 0.0072 0.0072 0.0072 0.0072 Niacinamide 0.004 0.004 0.004 0.004 0.004 D-Pantothenic 0.004 0.004 0.004 0.004 0.004 Acid, Ca Pyridoxal•HCl 0.004 0.004 0.004 0.004 0.004 Riboflavin 0.0004 0.0004 0.0004 0.0004 0.0004 Thiamine•HCl 0.004 0.004 0.004 0.004 0.004 Other: D-Glucose 1 Absent 1 4.5 Absent Pyruvic Acid, 0.11 0.11 Absent 0.11 Absent Sodium Phenol Red, 0.0159 0.0159 Absent 0.015 Absent Sodium Cloning of NOXes into pLVX-TRE3G Vector Human codon optimized genes encoding H2O-forming NADH oxidases (NOXes) from Lactobacillus brevis (SEQ ID NO: 316), Lactococcus lactis (SEQ ID NO: 317), Streptococcus pneumoniae (SEQ ID NO: 318), Streptococcus mutans (SEQ ID NO: 319), and Serpulina hyodysenteriae (SEQ ID NO: 320), flanked by NotI and MluI restriction sites in pUC57 vector, were custom synthesized by GENEWIZ. .. After digestion PCR product was ligated into pET30a vector (EMD Millipore). ..

    Cloning:

    Article Title: Hfe Permease and Haemophilus influenzae Manganese Homeostasis.
    Article Snippet: Haemophilus influenzae is a commensal of the human upper respiratory tract that can infect diverse host niches due, at least in part, to its ability to withstand both endogenous and host-mediated oxidative stresses.. Here, we show that hfeA, a gene previously linked to iron import, is essential for H. influenzae manganese recruitment via the HfeBCD transporter.. Structural analyses show that metal binding in HfeA uses a unique mechanism that involves substantial rotation of the C-terminal lobe of the protein.

    Article Title: A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’
    Article Snippet: .. The coding sequence of LoAAT1 was amplified using high-fidelity DNA polymerase KOD-Plus (TOYOBO) with the primers in and then constructed into the pET30a vector (Novagen) through the cloning sites Eco RI and Hind III. ..

    Modification:

    Article Title: Hfe Permease and Haemophilus influenzae Manganese Homeostasis.
    Article Snippet: Haemophilus influenzae is a commensal of the human upper respiratory tract that can infect diverse host niches due, at least in part, to its ability to withstand both endogenous and host-mediated oxidative stresses.. Here, we show that hfeA, a gene previously linked to iron import, is essential for H. influenzae manganese recruitment via the HfeBCD transporter.. Structural analyses show that metal binding in HfeA uses a unique mechanism that involves substantial rotation of the C-terminal lobe of the protein.

    Generated:

    Article Title: Hfe Permease and Haemophilus influenzae Manganese Homeostasis.
    Article Snippet: Haemophilus influenzae is a commensal of the human upper respiratory tract that can infect diverse host niches due, at least in part, to its ability to withstand both endogenous and host-mediated oxidative stresses.. Here, we show that hfeA, a gene previously linked to iron import, is essential for H. influenzae manganese recruitment via the HfeBCD transporter.. Structural analyses show that metal binding in HfeA uses a unique mechanism that involves substantial rotation of the C-terminal lobe of the protein.

    Expressing:

    Article Title: Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
    Article Snippet: .. A synthetic gene coding for M. hassiacum MmpH and sequence-optimized for expression in E. coli (GenScript) was cloned into the NdeI and HindIII restriction sites of the pET30a vector (Novagen) and C-terminal His6-tagged MmpH was overexpressed in E. coli BL21(DE3) cells. ..

    Article Title: Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
    Article Snippet: .. A synthetic gene coding for M. hassiacum MeT1 was sequenceoptimized (GenScript) for expression in E. coli, cloned into the NdeI and HindIII restriction sites of the pET30a vector (Novagen), and overexpressed in E. coli BL21(DE3) cells as a C-terminal His6-tagged MeT1. ..

    Clone Assay:

    Article Title: Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
    Article Snippet: .. A synthetic gene coding for M. hassiacum MmpH and sequence-optimized for expression in E. coli (GenScript) was cloned into the NdeI and HindIII restriction sites of the pET30a vector (Novagen) and C-terminal His6-tagged MmpH was overexpressed in E. coli BL21(DE3) cells. ..

    Article Title: Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides.
    Article Snippet: .. A synthetic gene coding for M. hassiacum MeT1 was sequenceoptimized (GenScript) for expression in E. coli, cloned into the NdeI and HindIII restriction sites of the pET30a vector (Novagen), and overexpressed in E. coli BL21(DE3) cells as a C-terminal His6-tagged MeT1. ..

    Construct:

    Article Title: A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’
    Article Snippet: .. The coding sequence of LoAAT1 was amplified using high-fidelity DNA polymerase KOD-Plus (TOYOBO) with the primers in and then constructed into the pET30a vector (Novagen) through the cloning sites Eco RI and Hind III. ..



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